|
KMID : 0363320070280010166
|
|
Journal of Korean Oriental Internal Medicine
2007 Volume.28 No. 1 p.166 ~ p.175
|
|
|
Neuroprotective Effect of Cirsium japonicum and Silibinin on Lipopolysaccharide-induced Inflammation in BV2 Microglial Cells
|
|
Yeo Hyun-Soo
Kim Dong-Woo
Jeon Chan-Yong
Choi You-Kyung
Park Jong-Hyung
|
|
|
Abstract
|
|
|
Objectives : This study was designed to evaluate the neuroprotective effect of Cirsium japonicum and Silibinin on lipopolysaccharide-induced inflammation in BV2 microglial cells.
Methods : We studied on the neuroprotective effect of lipopolysaccharide-induced inflammation using MTS assay, western blot, and nitric oxide detection on mouse BV2 microglial cells.
Results : Cirsium japonicum dose-dependently (50{mu}g/ml{sim}250{mu}g/ml) inhibited nitrite production and iNOS expression in lipopolysaccharide-induced BV2 microglia and also significantly reduced lipopolysaccharide-induced COX-2 activation in western blot. Silibinin dose-dependently (10{mu}M{sim}100{mu}M) inhibited nitrite production and iNOS expression in lipopolysaccharide-induced BV2 microglial cells. Silibinin also significantly reduced lipopolysaccharide-induced COX-2 activation in western blot.
Conclusion : These effects of neuroprotection related to anti-inflammation suggest that Cirsium japonicum and Silibininmay be useful candidates for the development of a drug for related neurodegenerative diseases.
|
|
|
KEYWORD
|
|
|
Cirsium japonicum, Silibinin, BV2 Microglial Cells, Oriental Herb-Medicine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|